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4 edition of Effects of temperature on desaturase enzyme gene expression in brassica napus leaves found in the catalog.

Effects of temperature on desaturase enzyme gene expression in brassica napus leaves

Rajesh Khetarpal

Effects of temperature on desaturase enzyme gene expression in brassica napus leaves

by Rajesh Khetarpal

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Published by National Library of Canada in Ottawa .
Written in English


Edition Notes

Thesis (M.Sc.) -- University of Toronto, 1998.

SeriesCanadian theses = -- Thèses canadiennes
The Physical Object
FormatMicroform
Pagination1 microfiche : negative. --
ID Numbers
Open LibraryOL19418731M
ISBN 100612406989
OCLC/WorldCa51086548

  Abstract. Perilla (Perilla frutescens var. frutescens) seeds contain approximately 40 % of oil, of which α-linolenic acid () comprise more than 60 % in seed oil and 56 % of total fatty acids (FAs) in leaf, perilla, endoplasmic reticulum (ER)-localized and chloroplast-localized ω-3 FA desaturase genes (PfrFAD3 and PfrFAD7, respectively) have already been reported, however 【Objective】To determine major fatty acid contents of progenies mutated by EMS,and analyze the high oleate mutant() obtained from M2 at molecular level.【Method】The rapeseed seeds were treated with EMS at there levels of %,% and %, fatty acid contents were analyzed in M1 and M2 individual plants of mutagenic FAD2 genes were cloned for the

Read "Identification of the Arabidopsis Palmitoyl-Monogalactosyldiacylglycerol Δ7-Desaturase Gene FAD5, and Effects of Plastidial Retargeting of Arabidopsis Desaturases on the fad5 Mutant Phenotype" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your :// acyl-lipid omega-6 desaturase (cytochrome b5) IUBMB Comments This microsomal enzyme introduces a cis double bond in fatty acids attached to lipid molecules at a location 6 carbons away from the methyl end of the fatty ://?ecno=

The enzyme from the fungus Mortierella alpina acts on gamma-linolenoyl and arachidonoyl groups, converting them into stearidonoyl and icosapentaenoyl groups, respectively. cf. EC , sn-2 acyl-lipid omega-3 desaturase (ferredoxin).?ecno= Carotenoids, the naturally occurring isoprenoids form essential components of photosynthetic antenna and reaction centre complexes. Thus they play a significant role in absorption, dissipation and transfer of light energy for the process of photosynthesis. The effects of salt stress on carotenoid gene expression in tomato leaves were ://


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Effects of temperature on desaturase enzyme gene expression in brassica napus leaves by Rajesh Khetarpal Download PDF EPUB FB2

Effects of temperature on desaturase enzyme gene expression in Brassica napus leaves. Rajesh Khetarpal Department of Botany, University of Toronto ABSTRACT A generally accepted characteristic of higher plants is their ability to adjust the fatty acid composition of their membranes in response to changing growth :// This system is highly expected to be further applied for the breeding of various agronomically important plant species.

Here we report the modification of a fatty acid desaturase 2 gene (FAD2), which encodes an enzyme that catalyzes the desaturation of oleic acid, in Brassica napus cv. Westar using the CRISPR   A global analysis of light and temperature dependent alterations of gene expression in B.

oleracea var. sabellica leaves was performed with the most comprehensive Brassica microarray. When compared to the light experiment much less genes were differentially expressed in kale leaves   The substrate specificity and regioselectivity of the Brassica napus extraplastidial linoleate desaturase (FAD3) was investigated in vivo in a heterologous expression system.

A strain of the yeast Saccharomyces cerevisiae producing the plant enzyme was constructed and cultured in media containing a variety of fatty acids. The products of desaturation of these potential substrates were CRISPR/Cas9-mediated genome editing of the gene FAD2 in B.

napus increased the content of OA [29]. Meanwhile, the loss of function mutations, EK and GE in BnFAD Strong artificial and natural selection causes the formation of highly conserved haplotypes that harbor agronomically important genes.

GWAS combination with haplotype analysis has evolved as an effective method to dissect the genetic architecture of complex traits in crop species. We used the 60 K Brassica Infinium SNP array to perform a genome-wide analysis of haplotype blocks associated with The expression cassette was fused to the SsSAD gene and the Basta-resistant selectable marker gene, which were driven by the CaMV35S promoter and expressed in rape plants (Fig.

3A). After the first screening with the herbicide, we obtained 22 independent lines (Fig. 3B).Three Basta-resistant independent lines with a segregation ratio of (35S:SsSAD-2, 35S:SsSAD-5, and 35S:SsSAD-9), Vegetable oils rich in oleic acid are more desirable than oils rich in polyunsaturated and saturated fatty acids.

The biological switch of oleic acid to linoleic acid is facilitated by fatty acid desaturase 2 enzyme that is further classified into FAD, FAD, FAD, and FAD The genes coding these enzymes have high sequence similarity, but differ mostly in their expression ://   Abstract.

Brassica napus (canola) plants were genetically manipulated to increase the amount and composition of carotenoids in seeds by using seven key enzyme genes involved in ketocarotenoid formation, which originated from a soil bacterium Pantoea ananatis (formerly called Erwinia uredovora 20D3), and marine bacteria Brevundimonas sp.

strain SD and Paracoccus sp. strain N   Hexadeca 7,10,trienoic acid (Δ7,10,13) is one of the most abundant fatty acids in Arabidopsis (Arabidopsis thaliana) and a functional component of thylakoid membranes, where it is found as an sn-2 ester of monogalactosyldiacylglycerol.

The Arabidopsis fad5 mutant lacks activity of the plastidial palmitoyl-monogalactosyldiacylglycerol Δ7-desaturase FAD5, and is characterized   Temperature is a major environmental factor that regulates fatty acid desaturation in plants. The regulation of gene expression appears to vary with the species, tissue, and gene.

The effect of temperature on the FAD gene expression levels was well studied in Fatty acid unsaturation, mobilization, and regulation in the response of plants to stress Robert G. Upchurch of plant defense gene expression (Kachroo et al.

) and seed colonization by fungi (Calvo et al. (Brassica napus) plants shows decreased mainly in the chloroplast monogalactosyldiacylglycerol Light intensity and temperature are very important signals for the regulation of plant growth and development.

Plants subjected to less favorable light or temperature conditions often respond with accumulation of secondary metabolites. Some of these metabolites have been identified as bioactive compounds, considered to exert positive effects on human health when consumed ://   Chimeric genes contribute to the evolution of diverse functions in plants and animals.

However, new chimeric genes also increase the risk of developmental defects. Here, we show that the chimeric gene Brassica napus male sterile 4 (Bnams4b) is responsible for genic male sterility in the widely used canola line A (Bnams3 ms3ms4bms4b)   Sun, M.

et al. Design of new genome-and gene-sourced primers and identification of QTL for seed oil content in a specially high-oil Brassica napus cultivar. Plos One 7, e (). ADS By contrast, expression of the regulatory gene CER2 is not induced by light, temperature, drought or other stress conditions, but may be induced by cytokinins in young leaves (Xia et al., ) or by specific trancription :// Characterization of the Brassica napus Extraplastidial Linoleate Desaturase by Expression in Saccharomyces cerevisiae Article (PDF Available) in Plant physiology (3) April with   All flowering plants have experienced repeated rounds of polyploidy (whole-genome duplication), which has in turn driven the evolution of novel phenotypes and ecological tolerances and been a major driver of speciation.

The effects of polyploidy on gene expression have been studied extensively at the level of transcription and, to a much lesser extent, at the level of the steady state   Zhang, D. et al. Identification and expression of a new delta fatty acid desaturase (FAD) gene in upland cotton and its functional expression in yeast and Arabidopsis thaliana plants   In the present study, we cloned the CsSAD gene, ana-lyzed its sequence characteristics, and studied its expression under low temperatures, abscisic acid (ABA) treatment, drought stress, and wounding at low temperature.

We also analyzed CsSAD expression in different tissues, and examined the effects of CsSAD expression in two. Introduction. Brassica napus () is the third largest source of plant oil after oil palm and soybean, producing approximately 15% of edible oil globally (Gracka et al., ; Liu et al., ).It is closely related to Arabidopsis sharing about 81% conserved sequence for most gene sequence (Parkin et al., ).Analysis indicates that the oil biosynthesis pathways are mostly conserved   The application and nutritional value of vegetable oil is highly dependent on its fatty acid composition, especially the relative proportion of its two major fatty acids, i.e oleic acid and linoleic acid.

Microsomal oleoyl phosphatidylcholine desaturase encoded by FAD2 gene is known to introduce a double bond at the Δ12 position of an oleic acid on phosphatidylcholine and convert it to This review focuses on the responses of the plant cell wall to several abiotic stresses including drought, flooding, heat, cold, salt, heavy metals, light, and air pollutants.

The effects of stress on cell wall metabolism are discussed at the physiological (morphogenic), transcriptomic, proteomic and biochemical levels. The analysis of a large set of data shows that the plant response is